Chromatography Peak Resolution & Plate Height Calculator

Determine chromatographic separation efficiency, USP peak resolution (Rs), plate count (N), and HETP per USP <621> and EP 2.2.46.

Peak Retention & Width Data

Peak 1 Retention Time tᵣ₁ (min):

Peak 1 Baseline Width w₁ (min):

Peak 2 Retention Time tᵣ₂ (min):

Peak 2 Baseline Width w₂ (min):

Void / Dead Time t₀ (min):

Column Length L (mm):

Separation Metrics & Efficiency

USP Resolution (Rs):

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Resolution Status:

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Theoretical Plates N₂:

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HETP (Plate Height):

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Retention Factor k'₂:

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Selectivity Factor (α):

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Frequently Asked Questions

What is the benchmark value for baseline resolution?

A peak resolution of Rs ≥ 1.5 represents true baseline resolution for adjacent Gaussian peaks with less than 0.15% peak area overlap.

How does plate height HETP correlate with column quality?

Lower HETP indicates higher column separation efficiency. In well-packed HPLC columns, HETP is approximately 2 to 3 times the particle diameter (dp).

What is the difference between selectivity (α) and resolution (Rs)?

Selectivity (α) measures thermodynamic chemical affinity difference between stationary phase and analytes, whereas resolution (Rs) combines selectivity, efficiency (N), and retention factor (k).