Electrophoretic Mobility & Gel Calculator
Bioanalytical engineering & molecular separation: Compute electrophoretic mobility ($\mu_e$), DNA/protein migration velocity, Ferguson plot retardation, and Joule heating dissipation.
Electrophoresis Chamber & Voltage
DC power supply setting
Inter-electrode gel tank distance
Gel Matrix & Biomolecule
e.g. 1.0% agarose or 12% polyacrylamide
DNA base pairs (bp) or protein kDa
Used for Joule heating power
Ferguson Plot & Ogston Sieving
The electrophoretic migration velocity is $v = \mu_e \cdot E$ where electric field $E = V / L$.
In a polymer gel, mobility is retarded according to the Ferguson relationship:
$$\log(\mu) = \log(\mu_0) - K_R \cdot \%T$$
Where $K_R$ is the retardation coefficient proportional to molecular size.
Migration Kinematics
Migration Velocity (v)
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Electric Field Gradient (E)
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Mobility & Thermal Dissipation
Apparent Mobility (μ_e)
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10⁻⁴ cm² / (V·s)
Joule Heating Power
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P = V · I (Watts)
Run Duration & Resolution
Time to Migrate 8 cm:
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Electric Field Evaluation:
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Joule Thermal Smiling Hazard:
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