Surface Plasmon Resonance SPR Calculator
Label-free optical biosensing: Calculate Kretschmann resonance angle (\(\theta_{\text{spr}}\)), resonance dip shift (\(\Delta \theta\)), bulk sensitivity, and analyte binding mass.
Kretschmann Prism & Sensor Film
BK7 glass = 1.515, SF11 = 1.78
Biacore standard: 670 nm
Aqueous buffer / PBS ~ 1.333
From analyte molecular binding
Optimal: ~ 48-50 nm Au
Au at 670 nm ~ -15.5
Proteins/antibodies ~ 0.185
Resonance Angle & Surface Mass Density
Baseline Resonance Angle (θ_spr)
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Resonance Angle Shift (Δθ)
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Bulk Sensitivity
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Surface Mass Density
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Decay Length L_z
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Biosensor Transduction Assessment
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