Sequence data

Stockholm gap-column trimmer

Remove alignment columns above a chosen unweighted gap fraction and export aligned FASTA.

Your inputs

Start with an example

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Your result

Example result

How it works

Treat dash and period as gaps. Retain columns whose gap count divided by sequence count is at or below the chosen maximum. Concatenate matching sequence blocks.

Columns 2 and 5 in the example have two gaps out of three sequences. At 0.5 they are removed, leaving ACG for each sequence.

Limits

Up to 2000 sequences and 100000 columns within the input limit. Output is aligned FASTA and omits Stockholm annotations. No sequence weighting, conservation model, fragment adjustment or recommended threshold is inferred.